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VOL. 10, ISSUE 3 (2026)
In vitro and in silico investigation of anti-inflammatory and hepatoprotective effects of Cordia macleodii leaf extract collected from Satna District, Madhya Pradesh
Authors
Roshni Singh, Dinesh Kumar Mishra, R S Nigam, Awanish Kumar Patel, Ashish Sohgaura
Abstract
*Cordiamacleodii* (Griff.) Hook. f.
& Thomson is an endangered ethnomedicinal plant traditionally used for
treating various ailments, including inflammatory conditions and hepatic
disorders. This study evaluates the anti-inflammatory and hepatoprotective
potential of *C. macleodii* leaf extract through in vitro assays, in
vivo-inspired models, and in silico molecular docking. Preliminary
phytochemical screening revealed high concentrations of total flavonoids (611.9
mg/g) and phenolics (164.4 mg/g), with tiliroside identified as a major
bioactive constituent. In vitro anti-inflammatory assays demonstrated a
dose-dependent inhibition of bovine serum albumin (BSA) denaturation, achieving
89% inhibition at 1000 µg/mL (IC50 ≈ 335 µg/mL), comparable to diclofenac
sodium. Hepatoprotective evaluation against carbon tetrachloride (CCl4)-induced
liver injury in rats showed that pretreatment with *C. macleodii* extract
(200–400 mg/kg) significantly reduced elevated serum enzymes (SGPT, SGOT, and
ALP) and total bilirubin, with the 400 mg/kg dose exhibiting efficacy nearly
identical to the standard drug silymarin. In silico molecular docking analysis
of tiliroside against human inflammatory and hepatic targets revealed strong
binding affinities: COX-2 (-8.4 kcal/mol), TNF-alpha (-7.2 kcal/mol), and
CYP2E1 (-7.9 kcal/mol). These findings suggest that the dual therapeutic
activity of *C. macleodii* is mediated through the modulation of oxidative
stress, enzyme inhibition, and cytokine down-regulation, validating its
traditional use and highlighting its potential as a source of novel hepatoprotective
and anti-inflammatory agents.
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Pages:1-7
How to cite this article:
Roshni Singh, Dinesh Kumar Mishra, R S Nigam, Awanish Kumar Patel, Ashish Sohgaura "<i>In vitro</i> and in silico investigation of anti-inflammatory and hepatoprotective effects of <i>Cordia macleodii </i>leaf extract collected from Satna District, Madhya Pradesh". International Journal of Chemistry Studies, Vol 10, Issue 3, 2026, Pages 1-7
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