ARCHIVES
VOL. 9, ISSUE 2 (2025)
Green synthesis and characterization of silver nanoparticles using Ocimum Sanctum leaf extract and evaluation of their antimicrobial activity
Authors
Yogasri G, Marx Nirmal R, Sivakumar G M, Selvan P
Abstract
To synthesize eco-friendly silver nanoparticles utilizing Ocimum
sanctum leaf extract, offering a sustainable alternative to chemical
synthesis methods and minimizing the need for microbial routes involving
bacteria and fungi. The present study was undertaken to assess the
effectiveness of green-synthesized silver nanoparticles (AgNPs). The
nanoparticles were prepared using Tulsi (Ocimum sanctum) leaf
extract, which served as both a reducing and stabilizing agent. The
biosynthesis was initiated by adding the leaf extract to a 0.01 M silver
nitrate solution, resulting in a rapid colour change from yellow to brown,
indicating nanoparticle formation, which was confirmed
through multiple characterization techniques. UV–Visible spectroscopy revealed
a characteristic peak at 413 nm, confirming nanoparticle formation. The
particle size was determined as 24.9 nm and zeta potential analysis indicated a
charge of −10 mV, reflecting good colloidal stability. EDX analysis confirmed
the elemental composition as 70.4% silver, while X-ray diffraction (XRD)
established a crystalline size of 14.71 nm with a face-centred cubic structure.
Scanning Electron Microscopy (SEM) showed spherical nanoparticles with smooth
surfaces. The AgNPs demonstrated strong antimicrobial activity against Escherichia
coli (16.10 ± 0.09 mm) and Salmonella typhimurium (17.20 ± 0.17 mm)
at 1 mg/mL concentration. The green-synthesized
silver nanoparticles showed excellent stability and significant antimicrobial
potential.
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Pages:45-52
How to cite this article:
Yogasri G, Marx Nirmal R, Sivakumar G M, Selvan P "Green synthesis and characterization of silver nanoparticles using <i>Ocimum Sanctum</i> leaf extract and evaluation of their antimicrobial activity". International Journal of Chemistry Studies, Vol 9, Issue 2, 2025, Pages 45-52
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