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VOL. 8, ISSUE 2 (2024)
Phytochemical screening and in vitro anti-breast cancer activity of Securidaca longependunculata root bark extracts on mcf-7 cells using mtt assay
Authors
L A Magashi
Abstract
Breast cancer remains a significant public
health concern, necessitating the exploration of novel therapeutic agents from
natural sources. This study evaluates the in vitro anti-breast cancer
activity of Securidaca longependunculata root bark extracts on MCF-7
breast cancer cells, utilizing the MTT assay to assess cell viability and
investigates the phytochemical constituents in the plant. The root barks of Securidaca
longependunculata was extracted, weight 100g with soxhlet extractor using
six different solvents based on polarity guided method and the respective
extracts were concentrated under reduced pressure. Percentage Yields and
Physico-Chemical Evaluation of the Extracts from the various fractions were
recorded, with highest yields from 70 % MeOH, Methyl acetate and MeOH fractions
and 25.513, 25.504 and 5.766 % respectively. The extracts exhibited significant
cytotoxic effects on MCF-7 cells, with an IC50 - 223.3 µg/ml and %
Inhibition of 64.62 in Methyl acetate and IC50 - 242 µg/ml with %
Inhibition of 61.22 in MeOH fractions compared to Oxaliplatin IC50
of 38.04µg/ml and % Inhibition of 74.96 µg/ml from their respective
concentrations, 320 µg/ml and 100 µg/ml. Phytochemicals were screened to
identify the active compounds responsible for the observed effects and results
revealed the presence of various bioactive compounds, including flavonoids,
alkaloids, Phenolics, Saponins and tannins. These findings reveals notable
cytotoxicity which may support the development of novel therapeutic agents
derived from this plant. This warrants further investigation into its bioactive
constituents and the development of novel breast cancer therapeutic agents from
the plant.
Pages:59-64
How to cite this article:
L A Magashi "Phytochemical screening and in vitro anti-breast cancer activity of Securidaca longependunculata root bark extracts on mcf-7 cells using mtt assay". International Journal of Chemistry Studies, Vol 8, Issue 2, 2024, Pages 59-64
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