Objectives: The present study involves in vitro antioxidant studies on stem part of Cissampelos pareira and the identification of active phytoconstituents present in methanolic extract of stem part of Cissampelos pareira by HPTLC method.

Materials and methods: Phytochemical studies were carried out using three different solvents like hexane, ethyl acetate and methanol. The antioxidant activity of different extracts was studied by in vitro assay using DPPH and IC₅₀ values were determined.Total phenolic and flavonoid contents and total antioxidant activity of methanolic extract of stem part of Cissampelos pareira were studied. By using HPTLC analysis the active phytoconstituents present in methanolic extract of stem part of Cissampelos pareira was also carried out.

Results: Preliminary phytochemical analysis revealed the presence of phenols, flavonoids, alkaloids, glycosides, saponins, steroids, carbohydrates, proteins and aminoacids. From the different extracts methanolic extract showed good antioxidant activity. HPTLC analysis of methanolic extract identified the presence of phenols and alkaloids.