Senna italica is easily distinguishable through its many distinctivefeatures.There are three subspecies in Sennaitalica based on the size of the inflorescence and the longth of the petiole. The subspecies are italic, micrantha and arachoides.In this work Senna italica seed oil was studied by GC-MS. The oil was also assessed for antimicrobial activity. The GC-MS analysis showed 19 components dominated by : 9,12-octadecadienoic acid methyl ester (40.27%) ; hexadecanoic acid methyl ester(20.06%) ; 9-octadecenoic acid methyl ester(17.32%) and methyl stearate (13.47%).The antibacterial activity of the oil was evaluated via the diffusion bioassay against five standard pathogenic bacteria (Gram positive: Staphylococcus aureus and Bacillus subtitlis; Gram negative : Esherichia coli and Pseudomonasa aeruginosa and the fungus Candida albicans ). The oil showed moderate antibacterial activity against Bacillus subtitlis.