This study investigated the concentrations of total petroleum hydrocarbons in sediments of the Kolo Creek, Ogbia of Bayelsa State, Nigeria. The study was carried out to assess the level of pollution of the Creek by collecting samples from four different stations within the creek. The samples were extracted with dichloromethane (DCM) using soxhlet extraction apparatus followed by subsequent clean up in column chromatography and analyzed using Gas Chromatography-flame ionization detector (GC-FID) for the determination of total petroleum hydrocarbons (TPH). The study revealed that the concentrations of total petroleum hydrocarbons ranged from 102.5112 to 126.4288 mg/kg in January, 114.2975 to 140.6604 mg/kg in March and 127.8051 to 160.4837 mg/kg in May while the mean levels ranged from 114.8711±12.397 to 142.5244±14.050 mg/kg during the months of investigation. The order of occurrence of total petroleum hydrocarbons in the stations were 4 > 2 > 1 > 3. The mean values of total petroleum hydrocarbons recorded in the Kolo Creek at the different stations were above the required limit in the sediments that is acceptable to FME of 30 mg/kg and also DPR of 50mg/kg although still far lower than the intervention value of 5,000mg/kg. The results indicated that the Ogbia axis of Kolo Creek is under pollution attack and hence the need for urgent steps for remediation to avert any serious environmental and health hazards that may arise as a result of the presence of total petroleum hydrocarbons.