The present study was done on P. amarus leaf extract in order to investigate the phytochemical screening as well as evaluation of its pharmacological (antibacterial, antioxidant, cytotoxicity bioassay) profiles. The phytochemical screening was done by color test and nuclear magnetic resonance spectroscopy. The antibacterial activities were determined by measuring the diameter of the zones of inhibition in millimeter. The cytotoxic potential of the test samples was determined by using brine shrimp lethality bioassay. The phytochemical screening revealed the presence of flavonoids, anthraquinones, terpenoids, steroids, coumarins, tannins, saponin, and reducing sugars in the crude extract of P. amarus leaves. The leaf extract displayed a remarkable antibacterial activity against all the tested bacteria. The extract also showed good scavenging activity having IC50 values 14.18 μg/mL. This study gives some concrete evidence to support the pharmacological use of P. amarus leaf extract as antimicrobial, antitumor or pesticidal activity.