In the present investigation, a Schiff base that produced by the condensation reaction of L-alanine with 2-hydroxyacetophenol as primary ligand (HL1) and 2-aminophenol as secondary ligand (L2) was synthesized and investigated. The synthesized Schiff base and 2-aminophenol ligands were used in the formation of three mixed ligand chelates with iron (III), cerium (IV) and thorium (IV) ions. The synthesized compounds were examined by applying analytical and physical tools. The bonding and electronic transitions confirmed the proper structures for Schiff base and mixed ligand chelates.