This study was aimed to extract seeds oil of Jatropha curcas and to identify the chemical constituents and to evaluate its potential antioxidant activity. The seeds were collected from South Kordofan state Sudan in December (2020). The extraction of seeds oil was carried out at Standards and Metrology Laboratories in (El-Obeid) and then analyzed at National Research Center and medical University for science and technology (Khartoum). 8 components of Jatropha curcas seeds oil extract were detected by Gas Chromatography-Mass spectroscopy (GC-MS) method. The results showed that the major components are: 9-octadecanoic acid methyl ester (39.24%); 9, 12-octadecanoic acid methyl ester (37.14%), hexadecanoic acid methyl ester (14.93%) and methyl stearate (8.08%). The antioxidant activity of the extracted oil was evaluated by using the standard 2, 2 diphenyl-1-picrylhydrazyl (DPPH) 0.5 ml. The antioxidant activity of the extracted oil was 19.94±1.39.