In this paper, antibacterial studies of newly synthesized β-ketoesters have been reported. Afetr the synthesis of β-ketoesters, antibacterial studies have been completed by using disc diffusion method. During the antibacterial studies the antibiotic ciprofloxacin was used as standard drug. The disc diffusion method for the antibacterial was used against four bacteria say E. coli (ATTC-25922), S. aureus (ATTC-25923), P. aeruginosa (ATTC-27853) and K. pneumonia.