Alkali soluble seeds polysaccharide was extracted from Quercus incana Roxb. on acid hydrolysis with sulphuric acid and obtained hydrolysate on paper chromatographic analysis led to separation of D-galactose and D-mannose in 1:5 molar ratio. Purified seeds polysaccharide was reduced after periodate oxidation with sodium borohydride and sulphuric acid by Smith degradation method. The obtained hydrolysate produced polyalcohols as glycerol and erythritol in 1:6 molar ratio by paper chromatography. The derivatives of polyalcohols were produced from seeds polysaccharide as glycerol-tri-O-p-nitrobenzoate and tetra-O-tosyl-erythritol. The absorbance of polyalcohols were recorded in photoelectrocolorimeter at 480mµ for glycerol and erythritol.