In this present study, we examined the species composition and natural diversity according to family wise, of tree species of different sites of semi-arid zone of Agra (U.P.). fabaceae family emerged as a dominant family was determined by identifying with all respected sites (Taj Nature Walk, Shajahan Garden, Paliwal Park, Company Garden and Mau Forest). In this study maximum family composition were found at site 2 Shajahan Garden (26%). We figured out the minimum number of tree species belong to rhamnaceae, capparaceae and bignoniaceae and minimum number of tree species belong to fabaceae family. During this study thirteen families and total twenty-five tree species were found at all recognized sites. Throughout the time of research period, we investigated the percentage of the natural regeneration was 20% fabaceae.