A series of 2-[2,4-bis(aryl/alkylamino)thiazol-5-oyl]benzothiazole derivatives were synthesized from 1-aryl-3-(N,N’-diarylamidino)thioureas and 2-(2-bromoacetyl)benzothiazole with triethylamine. Their structures were established on the basis of IR,¹H NMR, ¹³C NMR and mass spectral analyses. All the synthesized compounds were screened for their antibacterial and antifungal potential. All the compounds showed significant activity against the microorganisms tested.