As the bacterial resistance is increasing to drugs in various diseases, new areas for drugs production are being explored by researchers. From this one of the area is Bacterial endophytes. In the present research, we isolated 16 endophytic bacterial species from Grass collected from different regions of Pune and screened for the presence of antimicrobial compounds against Shigella species by disc diffusion method. From 16 endophytic bacterial species RB10 showed highest antimicrobial activity against Shigella species. These RB10 endophytic bacterial species was further selected for Production of secondary metabolites by submerged fermentation method.