The plants contain several chemicals known as phytochemical constituents. Preliminary phytochemical screening was performed on ethanolic extract of Cucurbita pepo Linn. by standard methods. The phytochemical screening revealed the presence of reducing sugars, alkaloids, flavonoids, tannins, gums, glycosides and terpenoids. The findings of the study concluded that the leaves and stems extracts may have potential bioactive substances that may be used to discover new and most potent drugs.