Objective: The aim of present work was to develop an accurate, simple and cost effective UV spectrophotometric method for estimation of gatifloxacin. Method: This method was based on area under curve of UV spectrum between 316 – 346 nm and validation as per ICH guideline Q2 (R1). The method has followed linearity in the range of 5-25 μg /ml. The value of correlation coefficient was 0.999.Satiafactory value of percent relative standard deviation for the intraday and inter-day precision indicates that method was precise. Result: Result of the recovery studies (102%) shows accuracy of method. The method developed method can be used for routine estimation of gatifloxacin in bulk and dosage form. Conclusion: The developed method can be concluded as accurate, sensitive and precise and can be easily applied to the pharmaceutical formulation.