Vol. 2, Issue 4 (2018)
Studies on physiochemical, qualitative and quantitative phytochemical, analysis and antibacterial activity of Oxalis pes-caprae and Erodium glaucophyllum utilized as a traditional folk treatment from Al-Khums Libya
Author(s): Salem Mohamed Edrah, Afifa Omeman, Soad Belhage, Salem Ahmad Hassan
Abstract: The aqueous and ethanolic extracts of Oxalis pes-caprae and Erodium glaucophyllum leaves were phytochemicals, physiochemical and antibacterial studied where results of the phytochemical analysis revealed are rich in alkaloids, tannins and phenols, flavonoids, glycosides, terpenes and glycosides and present in height quantity of concentration in both extracts, while saponins, and steroids present in moderate concentration, whereas coumarins were present in low quantity of concentration, though antraquinone’s not present in both extracts. The results of pH values were 4 intended for Oxalis pes-caprae and 4.82 intended for Erodium glaucophyllum, though the per cent yields of a chemical constituent in the aqueous and ethanolic extracts of the plants were 60.4, 50, 78.2 and 38 % for Oxalis pes-caprae and Erodium glaucophyllum, respectively. While per cent’s yields for alkaloids, saponins and flavonoids in the aqueous and ethanolic extracts were 44, 53, 33, 47, 31 and 52 %, individually for Oxalis pes-caprae, in addition, 40, 36, 37, 32, 48 and 46 % for Erodium glaucophyllum, correspondingly. The physiochemical analysis revealed the results of ash content were 84.5 and 63.5 %, and the results of total ash values 84.5 and 63.5, acid insoluble ash 4.2 and 5.1, water-soluble ash 3.6 and 3.9, soluble in chloroform 2.6 and 2.8, soluble in petroleum ether soluble extractive value 9.6 and 8.3, while the losing on drying was 2 and 3.4 % (W/W) concerning the Oxalis pes-caprae and Erodium glaucophyllum, respectively. The results of the minerals were iron and phosphorus exists in highest amounts, while each of cobalt and sulphur exists in moderate’s values, though each of calcium and manganese exists in low quantities, concerning the Oxalis pes-caprae and Erodium glaucophyllum, respectively. The results of heavy metals revealed that presence of mercury, nickel, zinc and silver existed in both plants, while cobalt exists in Oxalis pes-caprae and not exist in Erodium glaucophyllum as well for nickel was not exist at all. The results of the potency of Oxalis pes-caprae extracts against the bacterial strains were the inhibition zones against the Staphylococcus aureus 8, 9 mm of 50, 100 % of the aqueous extract, and 5, 8 mm of 50 and 100 % of the ethanolic extract. And the results of the potency of Erodium glaucophyllum extracts against the bacterial strains were the inhibition zones in contradiction of the Escherichia coli were 9, 8 mm of 100 % of the aqueous and ethanolic extracts, and 7, 8 mm of 100 % of both extracts against Proteus vulgaris, while the Pseudomonas aeruginosa were 8, 7 mm of 100 % of both extracts, although the inhibition zones in contradiction of the Salmonella were 7 mm of 100 % of both extracts, though the inhibition zones in contradiction of the Klebsiella were 11 and 8 mm of 100 % of both extracts, respectively. On the other hand, there was a negative response of 25 % and almost of 50 % of both extracts against the bacterial strains while it was more effective with increasing of extracts concentrations, In general, there was a considerable response of 100 % extracts concentrations of both plants. From these the results of the above investigation that the selected leaves extracts were showing good antimicrobial activity, and principally of the concentrations 50 and 100 % aqueous and ethanol extract of both plants.