Vol. 2, Issue 1 (2018)
Comparative GC-MS analysis of ethanolic extract of Alternanthera philoxeroides collected from polluted and unpolluted site
Author(s): U Arul Pamila, S Karpagam
Abstract: To analyze and characterize the phytoconstituents of ethanolic extract of the aerial parts of an edible plant Alternanthera philoxeroides (Mart.) Griseb collected from polluted and unpolluted sites using gas chromatography-mass spectroscopy (GC-MS). The shade dried aerial parts of plant powder A. philoxeroides were extracted with ethanol and crude ethanolic extracts was obtained. The concentrated extract was subjected to GC-MS analysis to detect the phytoconstituents. The GC-MS analysis was performed by using Perkin Elmer Clarus 500 model with the software Turbomass ver 5.2. GC-MS plays a key role in the analysis of unknown component of plant origin. The GC-MS analysis shows different peaks with low and high molecular weight, determining the presence of many bioactive compounds. The isolation and identification of phytoconstituents is based on the peak area, retention time, molecular weight and molecular formula. From GC-MS analysis, ethanol extracts of A. philoxeroides from polluted site showed higher phytoconstituents when compared to the ethanol extract of A. philoxeroides from unpolluted site. The ethanolic extract of aerial parts of Alternanthera philoxeroides proved to be a reservoir of bioactive compounds identified by GC-MS which could prove effective in the treatment of various diseases.
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