Vol. 2, Issue 1 (2018)
NMR determination of bioactive compound Isolated from the Aerial parts of Vernonia Pauciflora (Willd.) Less
Author(s): MS Sallau, H Ibrahim, A Ekalu, JD Habila
Abstract: Vernonia pauciflora is a plant found in the North central Nigeria and is used in the traditional treatment of many diseases including pneumonia, tuberculosis, skin infection, meningitis, whooping cough, typhoid fever, headache, and diarrhoea. The result of the zones of inhibition of growth of the microorganisms ranged from 17-20 mm for n-hexane extract, 22-28 mm for ethyl acetate and 20-25 mm for the methanol extract. The Minimum Inhibition Concentration (MIC) result for n-hexane, ethyl acetate and methanol extracts ranged from 7.5-30 mg/mL while the Minimum Bactericidal Concentration/Minimum Fungicidal Concentration (MBC/MFC) result for the extracts ranged from 15-60 mg/mL. Extensive chromatographic separation of the ethyl acetate extract led to the isolation of compound ADT1. The structure of the compound was established as 3-oxolanyl acetate using spectral analysis including 1D and 2D NMR. The antimicrobial activity of the isolated compound as indicated by the zone of inhibition ranged from 22-29 mm against Methicilin Resistant Staphylococcus aureus (MRSA), Vancomycin Resistant enterococci (VRE), Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Candida albicans, and Candida krusei.